Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose
نویسندگان
چکیده
منابع مشابه
Improving adventitious shoot regeneration from cultured leaf explants of Petunia hybrida using thidiazuron
The effect of various concentrations of thidiazuron (TDZ) with or without 2.7 μM of α-naphthalene acetic acid (NAA) on adventitious shoot formation of two Petunia hybrida cultivars was studied. Seeds from ‘Daddy Blue’ and ‘Dreams White’ cultivars were germinated in vitro. Expanded leaves from both seedlings and regenerated shoots were taken as explants. Explants were placed on MS media supplied...
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Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described.
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Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...
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A procedure was developed for the formation and regeneration of protoplasts in Clostridium tertium. Although in the parent strain most of the protoplasts gave rise to L-form colonies, derivatives could be isolated which exhibited high regeneration frequencies.
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The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...
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ژورنال
عنوان ژورنال: Biology
سال: 2020
ISSN: 2079-7737
DOI: 10.3390/biology9080228